Sequential extraction of DNA and DNA-binding proteins from low cell numbers.

bandstab. If the desired level of purity had not been achieved from the first round of bandstab, it was possible to reamplify the target fragment from the gel used to check the yield and purity of the initial bandstab products. The bandstab approach can bypass the need for cloning PCR products and all associated work required to generate recombinants, check the insert size and prepare recombinant DNA. The bandstab technique may be used to obtain a pure template ready for direct DNA sequencing within hours of first visualizing the band on a gel, with considerable savings in time, labor and reagents.